Semester VI
Essentials of Plant Biochemistry
SOILS 321 · 3 (2+1) credit hours
Syllabus
Topic-wise syllabus
Theory
9 topics · 32 lectures
| No. | Topic | Lectures |
|---|---|---|
| Unit I | ||
| 1 | Biochemistry – Introduction and importance, Properties of water, pH and buffer | 2 |
| 2 | Plant cell and its components | 1 |
| 3 | Bio-molecules – Structure, classification, properties and function of carbohydrates, amino acids, proteins, lipids and nucleic acids | 4 |
| 4 | Enzymes: General properties; Classification; Mechanism of action; Michaelis and Menten and Line Weaver Burk equation and plots; Introduction to allosteric enzymes, use of enzymes | 5 |
| Unit II | ||
| 5 | Metabolic energy and its generation – Metabolism – Basic concepts, Glycolysis, Citric acid Cycle, Pentose phosphate pathway, oxidative phosphorylation | 5 |
| 6 | Fatty acid oxidation | 2 |
| 7 | Biosynthetic Pathways – Photosynthesis, Gluconeogenesis, nitrogen fixation, fatty acid and starch formation | 5 |
| Unit III | ||
| 8 | Regulation of metabolic pathways | 3 |
| 9 | Secondary metabolites, Terpenoids, Alkaloids, Phenolic and their applications in food and pharmaceutical industries | 5 |
Practical
11 topics
| No. | Topic |
|---|---|
| 1 | Preparation of standard solutions and reagents |
| 2 | Determination of pH |
| 3 | Qualitative tests of carbohydrates |
| 4 | Qualitative tests of amino acids |
| 5 | Qualitative/quantitative tests of secondary metabolites |
| 6 | Quantitative estimation of soluble sugars and starch |
| 7 | Estimation of protein by Kjeldahl method and Lowry's method |
| 8 | Preparation of mineral solution from ash |
| 9 | Estimation of fat by Soxhlet method |
| 10 | Determination of acid value, saponification value and iodine number |
| 11 | Estimation of ascorbic acid |
Study material
Notes
Hindi
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English
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