Semester VI
Agricultural Microbiology and Phyto-remediation
PPT 322 · 2 (1+1) credit hours
Syllabus
Topic-wise syllabus
Theory
14 topics · 18 lectures
| No. | Topic | Lectures |
|---|---|---|
| Unit I | ||
| 1 | Introduction to Microbiology: Definition, applied areas of Microbiology and Importance of Microbiology | 1 |
| 2 | History of Microbiology: Discovery of microorganisms, spontaneous generation theory, Germ theory of diseases, Immunization, fermentation, and origin of life | 2 |
| 3 | Bacteria: cell structure, chemoautotrophy, photo autotrophy and growth | 2 |
| 4 | Bacterial genetics: Genetic recombination- transformation, conjugation and transduction, genetic engineering | 1 |
| 5 | Soil Microbiology: Nutrient mineralization and transformation | 1 |
| Unit II | ||
| 6 | Air Microbiology: Phyllosphere microflora, Phylloplane microflora, microflora of floral parts etc. | 2 |
| 7 | Food Microbiology: Microbial spoilage and principles of food preservations, Food poisoning | 1 |
| 8 | Water Microbiology: Types of water, water microorganisms, and microbial analysis of water e.g. coliform test, Purification of water | 1 |
| 9 | Industrial Microbiology: Microbial products, Biodegradation, Biogas production, Biodegradable plastics etc. | 1 |
| 10 | Biological control: Microbial biopesticides for plant disease management | 1 |
| Unit III | ||
| 11 | Concepts of rhizosphere microbiology- Rhizodeposits-biochemical nature, release mechanism in rhizosphere, function | 2 |
| 12 | Carbon flow in rhizosphere, Rhizosphere microbiome residents and their roles | 1 |
| 13 | Potential of plant growth promoting rhizobacteria (PGPR) and endophytes on soil health and sustainability | 1 |
| 14 | Bioremediation of polluted soils using microbial mediators and Phytoremediation of polluted soils | 1 |
Practical
12 topics
| No. | Topic |
|---|---|
| 1 | Study of the microscope |
| 2 | Acquaintance with laboratory material and equipment |
| 3 | Microscopic observation of different groups of microorganisms: moulds (Fungi) |
| 4 | Direct staining of bacteria by crystal violet (Gram staining of bacteria) |
| 5 | Negative or indirect staining of bacteria by nigrosin |
| 6 | Study of phyllosphere and rhizosphere microflora |
| 7 | Measurement of microorganisms |
| 8 | Preparation of culture media |
| 9 | Isolation and purification of rhizospheric microbes |
| 10 | Isolation and purification of N-fixers |
| 11 | Isolation and purification of Nutrient solubilizers |
| 12 | Isolation and purification of Endophytes |
Study material
Notes
Hindi
Being added
English
Being added