Semester VII
Micro-propagation Technologies
EC 4116 · 4 (3+1) credit hours
Syllabus
Topic-wise syllabus
Elective course. Students choose five electives in Semester VII.
Theory
10 topics · 40 lectures
| No. | Topic | Lectures |
|---|---|---|
| Unit I | ||
| 1 | Introduction, History, Advantages and limitations | 4 |
| 2 | Types of cultures (seed, embryo, organ, callus, cell) | 4 |
| 3 | Stages of micro propagation | 4 |
| 4 | Axillary bud proliferation (Shoot tip and meristem culture, bud culture) | 4 |
| Unit II | ||
| 5 | Organogenesis (callus and direct organ formation) | 4 |
| 6 | Somatic embryogenesis | 4 |
| 7 | Cell suspension cultures | 4 |
| 8 | Production of secondary metabolites | 4 |
| Unit III | ||
| 9 | Somaclonal variation | 4 |
| 10 | Cryopreservation | 4 |
Practical
10 topics
| No. | Topic |
|---|---|
| 1 | Identification and use of equipment in tissue culture Laboratory |
| 2 | Nutrition media composition |
| 3 | Sterilization techniques for media, containers and small instruments |
| 4 | Sterilization techniques for explants |
| 5 | Preparation of stocks and working solution |
| 6 | Preparation of working medium |
| 7 | Culturing of explants: Seeds, shoot tip and single node |
| 8 | Callus induction |
| 9 | Induction of somatic embryos, regeneration of whole plants from different explants |
| 10 | Hardening procedures |
Study material
Notes
Hindi
Being added
English
Being added