Semester VII
Biotechnology of Crop Improvement
EC 4114 · 4 (3+1) credit hours
Syllabus
Topic-wise syllabus
Elective course. Students choose five electives in Semester VII.
Theory
26 topics · 30 lectures
| No. | Topic | Lectures |
|---|---|---|
| Unit I | ||
| 1 | Impact of Biotechnology on crop improvement and the perspective of society | 1 |
| 2 | Various biotechnological techniques available for crop improvement – Plant Tissue Culture, Genetic Engineering, Genome editing, Marker Assisted breeding and Genomic Selection | 2 |
| 3 | Biosafety regulations and their application in Agricultural Biotechnology | 1 |
| 4 | Somaclonal variation and its use in crop improvement | 1 |
| 5 | Embryo culture; anther/pollen culture | 1 |
| 6 | Somatic embryogenesis; artificial seeds | 1 |
| 7 | Techniques of protoplast culture, regeneration and somatic cell hybridization, achievements and limitations, utility in the improvement of crop plants | 1 |
| 8 | Direct and Indirect methods of gene transfer in plants - Agrobacterium-mediated gene transfer in dicots and monocots | 1 |
| 9 | Direct DNA delivery methods (microinjection, particle gun method, electroporation) | 1 |
| 10 | Gene targeting and Gene silencing techniques | 1 |
| Unit II | ||
| 11 | Introduction to siRNA and Micro RNA technology; construction of siRNA vectors | 1 |
| 12 | Principle and application of gene silencing | 1 |
| 13 | Creation of transgenic plants | 1 |
| 14 | Debate over GM crops | 1 |
| 15 | Introduction to methods of genetic manipulation in different model systems | 1 |
| 16 | Introduction to genome editing – Various tools of genome editing, CRISPR-Cas9 with specific emphasis on Indian regulations | 1 |
| 17 | Cloning genomic targets into CRISPR/Cas9 plasmids | 2 |
| 18 | Electroporation of Cas9 plasmids into cells | 1 |
| 19 | Purification of DNA from Cas9 treated cells and evaluation of Cas9 gene editing | 1 |
| Unit III | ||
| 20 | In vitro synthesis of single guide RNA (sgRNA) Using Cas9/sgRNA complexes to test for activity on DNA substrates | 2 |
| 21 | Evaluate Cas9 activity by T7E1 assays and DNA sequence analysis | 1 |
| 22 | Applications of CRISPR/Cas9 technology in crop plants | 1 |
| 23 | Marker Assisted Breeding and Genomic Selection: Introduction to various DNA-based markers and their use in marker-assisted breeding | 2 |
| 24 | Foreground Selection, Recombinant Selection and background Selection | 1 |
| 25 | Marker-assisted backcross breeding, marker-assisted selection – success stories | 1 |
| 26 | Introduction to Genomic Selection | 1 |
Practical
4 topics
| No. | Topic |
|---|---|
| 1 | Agrobacterium-mediated transformation in Tobacco – preparation of construct, transfer to binary vector, transform Agrobacterium, prepare explant |
| 2 | Inoculation and Co-cultivation, antibiotic based selection of putative transformants, validation using PCR |
| 3 | Genome editing - preparation of CRISPR/CAS construct, direct transfer to plant, analysis of the targets |
| 4 | Planning of a MABB programme – selection of parents, crossing strategies, marker analysis |
Study material
Notes
Hindi
Being added
English
Being added