Semester VI
Fundamentals of Agricultural Biotechnology
GPB 322 · 3 (2+1) credit hours
Syllabus
Topic-wise syllabus
Theory
27 topics · 31 lectures
| No. | Topic | Lectures |
|---|---|---|
| Unit I | ||
| 1 | Introduction to Plant Tissue Culture and Genetic Engineering: History | 1 |
| 2 | Cellular totipotency and cytodifferentiation | 1 |
| 3 | Callus culture, Single-cell/suspension culture and their applications | 1 |
| 4 | Organogenesis and somatic embryogenesis | 1 |
| 5 | Somaclonal variation and its use in crop improvement | 1 |
| 6 | Embryo rescue technique and its significance in hybrid development | 1 |
| 7 | In-vitro fertilization, ovule culture and its significance in hybrid development | 2 |
| 8 | Protoplast isolation, culture and regeneration | 1 |
| 9 | Somatic hybridization (somatic hybrids and cybrids) and its application in crop improvement | 1 |
| 10 | Anther and pollen culture for haploid production | 1 |
| Unit II | ||
| 11 | Development of disease-free (virus free) plants through apical meristem culture | 1 |
| 12 | Micropropagation technique for the generation of quality planting material | 1 |
| 13 | Synthetic seeds and its applications | 1 |
| 14 | National certification and Quality management of Tissue Culture plants - secondary metabolite production - in vitro germplasm conservation | 1 |
| 15 | Introduction to Molecular Biology: DNA structure and function | 1 |
| 16 | DNA replication, transcription and translation, RNA, types and function | 2 |
| 17 | Structure of prokaryotic and eukaryotic gene | 1 |
| 18 | Central dogma of life - DNA replication, transcription, genetic codes, translation and protein synthesis | 1 |
| 19 | Lac Operon concept - Nucleic acid hybridization | 1 |
| 20 | DNA sequencing – Sanger method | 1 |
| Unit III | ||
| 21 | PCR (Polymerase chain reaction) and its applications | 1 |
| 22 | Introduction to recombinant DNA technology: DNA modifying enzymes and vectors | 1 |
| 23 | Plant genetic transformation – physical (Gene gun method), chemical (PEG mediated) and Agrobacterium-mediated gene transfer methods | 2 |
| 24 | Transgenic and its importance in crop improvement with successful stories | 1 |
| 25 | Biosafety issues in biotechnology | 1 |
| 26 | Introduction to various molecular markers: RFLP, RAPD, SSR, SNP etc. | 2 |
| 27 | Marker-assisted breeding in crop improvement | 1 |
Practical
14 topics
| No. | Topic |
|---|---|
| 1 | Introduction to Plant Tissue Culture Laboratory |
| 2 | Good Laboratory Practices |
| 3 | Media Preparation and sterilization |
| 4 | Glassware sterilization |
| 5 | Micropropagation, Anther culture, Apical meristem culture |
| 6 | Callus induction and culture |
| 7 | Preparation of synthetic seeds |
| 8 | Isolation of plasmid DNA |
| 9 | Quantification of DNA |
| 10 | Agarose Gel Electrophoresis and visualization of plasmid DNA |
| 11 | Restriction digestion of plasmid DNA and agarose gel electrophoresis |
| 12 | Isolation of Plant genomic DNA; PCR amplification of DNA |
| 13 | Gel electrophoresis of amplified DNA |
| 14 | Visit to tissue culture units/biotech labs |
Study material
Notes
Hindi
Being added
English
Being added